Biotin

Biotin
General
Systematic name
Chemical formula	C10H16N2O3S
Molecular weight	244.31 g/mol
Other names	Vitamin B7 Vitamin H
Vitamin properties
Solubility	Water
RDA (adult male)	30 µg/day
RDA (adult female)	30 µg/day
RDA upper limit (adult male)	None
RDA upper limit (adult female)	None
Deficiency symptoms	Dry scaly skin Fatigue Loss of appetite Nausea and vomiting Mental depression Tongue inflammation High cholesterol
Excess symptoms	None
Dietary sources	Liver and kidney Dairy products oysters lobster salmon Yeast Cauliflower Chicken breast Egg yolk
Infobox disclaimer and references

Vitamin H redirects here. In medical slang, Vitamin H may also refer to haloperidol.

Biotin, also known as vitamin H or B₇, is a vitamin that is important in the catalysis of essential metabolic reactions to synthesize fatty acids, in gluconeogenesis (generation of glucose from non-sugar carbon substrates like pyruvate, glycerol, and amino acids), and to metabolize leucine. Vitamins are organic (carbon-containing) nutrients obtained through the diet and essential in small amounts for normal metabolic reactions in humans.

Biotin is one of the B vitamins (vitamin B complex), a group of chemically distinct, water-soluble vitamins that also includes thiamine, riboflaven, niacin, pantothenic acid, pyridoxine, folic acid, and others. Once considered to be a single vitamin, vitamin B is now seen as a complex of different vitamins that generally are found in the same foods.

Biotin has the chemical formula C₁₀H₁₆N₂O₃S and is composed of an ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring.

General overview

Biotin is used in cell growth, the production of fatty acids, and metabolism of fats and amino acids. It plays a role in the Krebs Cycle, which is the process in which energy is released from food. Biotin not only assists in various metabolic chemical conversions, but also helps with the transfer of carbon dioxide. Biotin is also helpful in maintaining a steady blood sugar level.

Biotin is often recommended for strengthening hair and nails. Consequently, it is found in many cosmetic and health products for the hair and skin.

Uses

Biotin supplements are often recommended as a natural product to counteract the problem of hair loss in both children and adults. There are, however, no studies that show any benefit in any case where the subject is not actually biotin deficient. The signs and symptoms of biotin deficiency include hair loss that progresses in severity to include loss of eye lashes and eye brows in severely deficient subjects. Some shampoos are available that contain biotin, but it is doubtful whether they would have any useful effect, as biotin is not absorbed well through the skin.

Children with a rare inherited metabolic disorder called phenylketonuria (PKU; in which one is unable to break down the amino acid phenylalanine) often develop skin conditions such as eczema and seborrheic dermatitis in areas of the body other than the scalp. The scaly skin changes that occur in people with PKU may be related to poor ability to use biotin. Increasing dietary biotin has been known to improve seborrheic dermatitis in these cases.

People with type 2 diabetes often have low levels of biotin. Biotin may be involved in the synthesis and release of insulin. Preliminary studies in both animals and people suggest that biotin may help improve blood sugar control in those with diabetes, particularly type 2 diabetes.

Biotin deficiency

Biotin deficiency is a rare metabolic genetic disorder. For that reason, statutory agencies in many countries (e.g., the Australian Department of Health and Aging) do not prescribe a recommended daily intake. Biotin deficiency can have a very serious, even fatal, outcome if it is allowed to progress without treatment. Signs and symptoms of biotin deficiency can develop in persons of any age, race, or gender.

Biotin deficiency rarely occurs in healthy individuals, since the daily requirements of biotin are low, many foods contain adequate amounts, intestinal bacteria synthesize small amounts, and the body effectively scavenges and recycles biotin from bodily waste. However, deficiency can be caused by excessive consumption of raw egg-whites over a long period (months to years). Egg-whites contain high levels of avidin, a protein that binds biotin strongly. Once a biotin-avidin complex forms, the bond is essentially irreversible. The biotin-avidin complex is not broken down nor liberated during digestion, and the biotin-avidin complex is lost in the feces. Once cooked, the egg-white avidin becomes denatured and entirely non-toxic.

Initial symptoms of biotin deficiency include:

1. Dry skin
2. Seborrheic dermatitis
3. Fungal infections
4. Rashes including erythematous periorofacial macular rash
5. Fine and brittle hair
6. Hair loss or total alopecia

If left untreated, neurological symptoms can develop, including:

1. Mild depression, which may progress to profound lassitude and, eventually, to somnolence
2. Changes in mental status
3. Generalized muscular pains (myalgias)
4. Hyperesthesias and paresthesias

The treatment for biotin deficiency is to simply start taking some biotin supplements.

Biochemistry

Biotin is a cofactor responsible for carbon dioxide transfer in several carboxylase enzymes:

• Acetyl-CoA carboxylase alpha
• Acetyl-CoA carboxylase beta
• Methylcrotonyl-CoA carboxylase
• Propionyl-CoA carboxylase
• Pyruvate carboxylase

The attachment of biotin to various chemical sites, called biotinylation, can be used as an important laboratory technique to study various processes including DNA transcription and replication. Biotin itself is known to biotinylate histones, but is not found naturally on DNA.

Biotin binds very tightly to the tetrameric protein streptavidin, with a dissociation constant K_d in the order of 10^-15 mol/L (Bonjour 1977, Green 1975) or 4x10^-14 (Holmberg et al. 2005). Holmberg et al. (2005) note that the biotin-streptavidin system is the strongest noncovalent biological interaction known. This is often used in different biotechnological applications. Holmberg et al. showed how to utilize high temperatues to efficiently break the interaction without denaturation of the streptavidin.

In the biology laboratory, biotin is sometimes chemically linked, or tagged, to a molecule or protein for biochemical assays. The specificitiy of the biotin-streptavidin linkage allow use in molecular, immunological, and cellular assays (Holmberg et al. 2005). Since avidin and streptavidin bind preferentially to biotin, biotin-tagged molecules can be extracted from a sample by mixing them with beads covered with avidin or strepavidin, and washing away anything unbound to the beads.

For example, biotin can be tagged onto a molecule of interest (eg. protein), and this modified molecule will be mixed with a complex mixture of proteins. Avidin or streptavidin beads are added to the mixture, and the biotinylated molecule will bind to the beads. Any other proteins binding to the biotinylated molecule will also stay with the beads. All other unbound proteins can be washed away, and the scientist can use a variety of methods to determine which proteins have bound to the biotinylated molecule.

Biotinylated antibodies are used to capture avidin or streptavidin in both the ELISPOT and ELISA techniques.

References

ISBN links support NWE through referral fees

• The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures, Holmberg, A. et al Electrophoresis 2005, 26(3), 501-10.

Holmberg A, Blomstergren A, Nord O, Lukacs M, Lundeberg J, Uhlen M.

• Article on Biotin deficiency by Dr. Howard R Sloan

Bonjour 1977, Bonjour, J. R. 1977. Biotin in man's nutrition and therapy: A review. Int. J. Vitam. Nutr. Res. 47:107.

Green 1975 Adv Protein Chem. 1975;29:85-133. Related Articles, Links Avidin. Green NM.

Holmberg et al 2005).

See also

• Biotinylation
• Streptavidin

External links

• Jane Higdon, "Biotin", Micronutrient Information Center, Linus Pauling Institute
• Biotin - Biocytin (Brewer's YeastBiotin Complex)

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